1. 50 ?g/mL of anti-IgM F(ab')2 fragment (anti-BCR). (B) Purified CD23+ B cells from C57Bl/6 (WT) and Bcl-xL Transgenic (Tg) mice were stimulated in control and Ca;Mfi � Sem;WT and STIM DKO B cells stimulated with 0,1920
2. Plotted are the percentage of Live/Dead dye-excluding cells (mean � SD) from triplicate wells at indicated timepoints after anti-BCR stimulation. (C) Cell viability (mean � SD of triplicate wells) after 20 hours of stimulation in the absence and presence of caspase 8 selective inhibitor, z-IETD-fmk (100?M). (D) Purified CD23+ B cells from C57Bl/6, Rip3k -/-, and Rip3k -/-Casp8 -/-mice were stimulated for 16 hours in normal or EGTA (0.5 mM) buffered media and viability calculated as the fraction of Live/Dead dye-excluding cells;mM EGTA) media for 24 hours with anti-BCR (10 ?g/mL)
3. 2 fragment (anti-BCR) for indicated times in the absence or presence of Calcineurin (Cn) inhibitors FK506 (1 ?M) or Cyclosporine A (CsA, 0.2 ?M). (B) Bcl2l1 mRNA was measured in WT B cells after 3 hours of stimulation with anti-BCR in the presence and absence of extracellular Ca 2+ and Cn inhibitors (mean � 95% confidence interval, *statistically significant). (C) Bcl-xL protein expression was quantified at 20 hours after anti-BCR stimulation in the presence or absence of Cn inhibitors. (D) B cell viability measured at 24 hours anti-BCR stimulation in the presence and absence of Cn inhibitors (mean � SD, *p<0.05) (E) cRel protein expression was quantified 20 hours after anti-BCR stimulation in the presence or absence of Cn inhibitors. (F) c-Rel expression;C57Bl/6 (WT) mice stimulated with 10 ?g/mL anti-IgM F(ab')