1. Following incubation cells were washed with PBS containing 0.5% BSA and 2mM EDTA and anti-CCR7 BV 421 (BioLegend, Clone:G043H7, RRID:AB_11203894, 1:20 dilution) was added directly to the cell pellet and incubated for 30 min at 37�C followed by the remaining surface stain for 30 min at RT in the dark. Cells were then fixed/permeabilized (FIX & PERM � Cell Permeabilization Kit, Invitrogen) and stained with intracellular antibodies for 30 min RT;Antibodies used were: LIVE/DEAD Aqua (Invitrogen, Cat# L34965, 1:200 dilution
2. Clone: JES3-19F1, RRID:AB_397227, 1:6.25 dilution), anti-IFN?;Biolegend;Clone:RPA-T4, RRID:AB_2632791, 1:50 dilution), anti-CD8A BV785 (BioLegend, Clone:RPA-T8, RRID:AB_2563264, 1:50 dilution), anti-CXCR5 BV711 (BioLegend, Clone:J252D4, RRID:AB_2629526, 1:50 dilution), anti-Vd2 FITC (BioLegend, Clone:B6, RRID:AB_1089230, 1:50 dilution), anti-IL-10 PE
3. VAR2CSA-Mediated Host Defense Evasion of Plasmodium falciparum Infected Erythrocytes in Placental Malaria;A Tomlinson;Front Immunol,2023