1. UAS-IP3-sponge.m49 was provided by Dr. Andrew Frank (University of Iowa) and originally generated by Dr. Daisuke Yamamoto 37 . Two-photon Laser Injury and Imaging -Calcium Imaging Assay Drosophila embryos were collected on grape plates with yeast paste for four hours to synchronize the age of the animals. After 72h after egg laying (AEL), larvae were individually immobilized on agarose pads sandwiched between a slide and a coverslip using glycerol as the mounting media. Dendrites or axons were imaged and severed from da neurons using a MaiTai two-photon 900nm laser mounted on a Zeiss 780 LSM microscope using ZEN Black's bleaching and regions functions. A bleaching power of ~780mW was used on a 10x10 ROI focused on either a dendrite or an axon. Bleaching window settings were as follows: start bleaching after 30 scans, 20 iterations, different scan speed of 5 (pixel dwell time 12.61𝜇sec), safe bleach for GaAsP on, 900nm laser at 65%. Neurons were imaged for ~3 minutes at 0.242fps (740 frames), and injuries occurred approximately 7.25 seconds into the imaging session (frame 30). For each animal, one to two neuron(s) from abdominal segments 2-5 were injured and imaged before the animal was subsequently returned to individual housing;* ; Uas-Pkd Gfp;BDSC #94614), y 1 , w * ; UAS-PKD.KD.GFP (BDSC #94615), y 1 , w * ; UAS-PKD.SE.GFP (BDSC #94616)
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4. Nasally delivered VEGFD mimetics mitigate stroke-induced dendrite loss and brain damage;D Mauceri;Proc. Natl. Acad. Sci. U. S. A,2020