1. 6�10 9 PFU/ml, 0.5?l/site) was injected into three points of the ventricular wall of the apex. After euthanasia, the heart, the brain, and the SCG tissues were immediately harvested and fixed in paraformaldehyde, then they were frozen and cut into 5-um slices. Virus-EGFP was directly observed by fluorescence microscopy as before. All protocols are required to protect the samples from light. Statistical analysis Use GraphPad Prism software 8.0 (Inc. San Diego, CA) to draw the graphs and analyze the data. All data were presented as the mean � standard error. Comparison among three or four groups was evaluated by one-way ANOVA variance analysis and Tukey's multiple comparisons test to identify significant differences. Comparison between two groups was analyzed by unpaired Student's t-test (two-tailed). The normal distribution was also assessed via the Shapiro-Wilk normality test. Statistical significance was defined as P-value;Genomes Genes;PRV (PRV-CAG-RFP,1972
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