Author:
Zhao Fangzhu,Joyce Collin,Burns Alison,Nogal Bartek,Ramos Alejandra,Biddle Trevor,Pauthner Matthias,Nedellac Rebecca,Qureshi Huma,Mason Rosemarie,Landais Elise,Ward Andrew B.,Burton Dennis R.,Sok Devin
Reference97 articles.
1. 2020) to quality 705 trim, remove adapters and merge paired sequences. Sequences were then annotated with Abstar 706 in combination with UMI based error correction by AbCorrect;Joyce;707 Resulting annotated consensus sequences were deposited to MongoDB and Spark databases 708 for querying and data analysis in python on Jupyter and Zeppelin Notebooks
2. Another 3-4 uL of stain was applied to the 718 grid for 30-60 s, followed by blotting. Air-dried grids were stored under RT until ready for 719 imaging. Tecnai T12 electron microscope was used to collect image data at 120 kV and 52,000x 720 magnification. Leginon software was used for image collection (PMID: 15890530). Appion 721 software was used to pick particles from raw images, followed by placement of particles into 722 stacks and 2D reference-free alignment via MSA/MRA (PMID: 19263523);Monoclonal Fabs were incubated with BG505 SOSIP.664 trimer (0.3 mg/mL) in 6X molar excess 715 overnight in TBS buffer at RT
3. A novel assay for antibody-dependent cell-mediated cytotoxicity against HIV-1-or SIV-infected 729 cells reveals incomplete overlap with antibodies measured by neutralization and binding assays;M D Alpert;J. Virol,2012
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