1. The Jackson Laboratory), two 1060 weeks after injection with rAAV1-CAG-FLEX-ArchT-GFP;Mice were deeply anesthetized with
2. The isolated whole brains were 1062 immersed in ice-cold carbogenated NMDG ACSF solution (92 mM N-methyl D-glucamine, 2.5 1063 mM KCl, 1.25 mM NaH2PO4, 30 mM NaHCO3, 20 mM HEPES, 25 mM glucose, 2 mM thiourea, 5 1064 mM Na-ascorbate, 3 mM Na-pyruvate, 0.5 mM CaCl2, 10 mM MgSO4;Euthasol (0.06 ml/30g), decapitated, and the brain removed
3. Parasagittal cerebellar brain slices 300 ?m) were cut using a 1066 vibratome;NMDG ACSF at 34�C 1067 for 15 minutes, and transferred into a holding solution of HEPES ACSF (92 mM NaCl, 2.5 mM,1200