Author:
Andreska Thomas,Lüningschrör Patrick,Ip Chi Wang,Rattka Marta,Siegl Christine,Perschin Veronika,Blum Robert,Aufmkolk Sarah,Sauer Markus,Monoranu Camelia Maria,Volkmann Jens,Stigloher Christian,Sendtner Michael
Subject
General Earth and Planetary Sciences,General Environmental Science
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1. Ethyleneglycol) at -20�C. Antibodies for immunostaining The following primary antibodies were used for immunocytochemical (ICC) or immunohistochemical (IHC) detection: Rabbit anti RFP (Rockland Cat# 600-401-379;Mouse pups (P1-5) were not perfused, but brains were incubated in 4% PFA at 4�C for 8-12h o.n. Brains were then washed in 1xPBS and embedded in 6% agarose. 20-40�m free-floating, coronal brain sections were obtained, using a LEICA Vibratome VT1000S (RRID:SCR_016495) and stored in "Cryoprotection Anti-Freeze Buffer
2. Following secondary antibodies were used for WB detection. Donkey anti Goat POD (Jackson ImmunoResearch Labs Cat;Alexa647 (Jackson Immunoresearch; 711-605-152, RRID: AB_2492288), DyLight649 (Jackson Immunoresearch; 711-495-152, RRID: AB_2315775)
3. Motor cortex is required for learning but not for executing a motor skill;R Kawai;Neuron,2015
4. A region in the dorsolateral striatum of the rat exhibiting single-unit correlations with specific locomotor limb movements;M O West;J Neurophysiol,1990