Molecular Architecture of Androgen Receptor Pathways Amplifying Glucagon-Like Peptide-1 Insulinotropic Action in Male Pancreatic β-Cells

Author:

Xu Weiwei,Qadir Fahd,Nasteska Daniela,Mota de Sa Paula,Gorvin Caroline M.,Blandino-Rosano Manuel,Evans Charles R.,Ashford Fiona B.,Bitsi Stavroula,Fan Jia,Bhondeley Manika,Song Kejing,Sure Venkata N.,Schiffer Lina,Beatty Wandy,Wyatt Rachael,Frigo Daniel E.,Liu Xiaowen,Katakam Prasad V.,Arlt Wiebke,Buck Jochen,Levin Lonny R.,Hu Tony,Kolls Jay,Burant Charles F.,Tomas Alejandra,Bernal-Mizrachi Ernesto,Hodson David,Mauvais-Jarvis Franck

Publisher

Elsevier BV

Subject

General Earth and Planetary Sciences,General Environmental Science

Reference75 articles.

1. All relative protein level data points were normalized for protein loading and transformed to linear values. Protein levels were further normalized by GAPDH and ?-actin. Enrichment (fold-change) between treatments and control group were reported for all phosphoproteins of interest. For heatmap representation proteins were normalized via a Z-test in R using customized scripts and plotted using the ggplot2 and heatmap.2 packages. Single cell RNA sequencing. Human islets (750 IEQ per condition) were treated with either 10nM DHT or EtOH overnight in a humidified incubator containing 4% CO2 at 37�C. Islet cells were then dispersed using TrypLE (Thermofischer), and immediately evaluated for viability (89.88�1.77%) by Cellometer Automated Cell Counter (Nexcelom Bioscience) prior to single cell RNAseq library preparation. For 10x single cell RNAseq library preparation, 5000 individual live cells per sample were targeted by using 10x Single Cell 3' RNAseq technology provided by 10x Genomics;R Perez;Human islets (1200 IEQ/condition) were cultured overnight and treated with DHT,2009

2. Overexpression of Kinase-Dead mTOR Impairs Glucose Homeostasis by Regulating Insulin Secretion and Not beta-Cell Mass;E U Alejandro;Diabetes,2017

3. Skeletal muscle-specific ablation of raptor, but not of rictor, causes metabolic changes and results in muscle dystrophy;A Benavides-Serrato;Cell metabolism,2008

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5. Rho GTPases and their effector proteins;A L Bishop;Biochem J 348 Pt,2000

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