1. C-terminal telopeptide of type I collagen (CTX-I) (ZC-35983, ZCI BIO, China), tartrate-resistant acid phosphatase 5b (TRACP-5B) (ZC-55533, ZCI BIO, China), follistatin-like 3 (FSTL3) (ZC-55528, ZCI BIO, China), and inositol 1,4,5-trisphosphate receptor type 2 (ITPR2) (ZC-55529, ZCI BIO, China) were determined using ELISA kits according to the manufacturer's instructions. The optical density of the samples was read using a microplate reader;Germany Roche;ZCI BIO, China), alkaline phosphatase (ALP) (ZC-36805, ZCI BIO, China), osteocalcin (OCN) (ZC-36660, ZCI BIO, China), procollagen type I N-terminal propeptide (PINP) (ZC-36129, ZCI BIO, China)
2. Collaboration; CKD (2020) Global, regional, and national burden of chronic kidney disease, 1990-2017: a systematic analysis for the Global Burden of Disease Study;Lancet,2017
3. Prevalence of chronic kidney disease in China: a cross-sectional survey;L Zhang;Lancet,2012