1. ) provided the dialysis tubing with molecular weight cutoff;ThermoFisher Scientific
2. Deionized (DI) water Modulab 2020 water purification system;Sephacryl Da;GE Healthcare
3. KNS42 was kindly provided by Dr. Chris Jones, The Institute of Cancer Research, London. NP53 was derived from a tumor arising in a DIPG genetically engineered mouse model induced by PDGF-B signaling, TP53 loss, and ectopic H3.3K27M expression.[33] NP53 cells were kindly provided by Dr;Robert H Ann;H35 cells were cultured in DMEM media supplemented with N2, 5% fetal bovine, 20ug/ml bovine pituitary extract 40 ng/ml of EGF and 20ng/ml bFGF. SJ-GBM2, KNS42 and U87 were cultured in maintained in RPMI media