Square Wave Voltammetric Approach to Leptin Immunosensing and Optimisation of Driving Parameters with Chemometrics

Author:

Shukla Shubhangi,Riley Parand R.,Joshi Pratik,Narayan Roger

Publisher

Elsevier BV

Subject

General Earth and Planetary Sciences,General Environmental Science

Reference48 articles.

1. APTMS stabilized AuNPs were synthesized by reduction of a gold precursor in the presence of an organic reducing agent;APTMS capped gold nanoparticles,2016

2. The rabbit anti-leptin monoclonal Abs (2-4 ?L) solution was added to the micellar AuNP suspensions and incubated for 1 h at 4�C to allow for direct adsorption via residual groups. Figure S1d shows the appearance of consistent rings on all of the particles after Abs loading, confirming the peripheral attachment of Abs molecules. After incubation, the suspensions of micellized AuNPs/Abs assemblies were centrifuged for 5-10 minutes at 4000 rpm. The obtained residues containing AuNP/Ab arrays were resuspended in 20 ?L of PBS each. The process was repeated two times to remove excess Ab molecules. The electrochemical immunoassays were performed by varying the concentration of Ab solutions (1.1 mg/mL-0.0001 ng/mL);Briefly;30 ?L of APTMS

3. Response surface methodology based on central composite design as a chemometric tool for optimization of dispersive-solidification liquid–liquid microextraction for speciation of inorganic arsenic in environmental water samples

4. Use of Response Surface Methodology To Develop and Optimize the Composition of a Chitosan–Polyethyleneimine–Graphene Oxide Nanocomposite Membrane Coating To More Effectively Remove Cr(VI) and Cu(II) from Water

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