Delayed Booster Dosing Improves Human Antigen-Specific IG and B Cell Responses to the RH5.1/AS01 <sub>B</sub> Malaria Vaccine

Author:

Nielsen Carolyn M.,Barrett Jordan R.,Davis Christine L.,Fallon Jon K.,Goh Cyndi,Michell Ashlin R.,Griffin Catherine L.,Kwok Andrew,Loos Carolin,Darko Samuel,Laboune Farida,Silk Sarah E.,Tekman Mehmet,Francica Joe,Ransier Amy,Payne Ruth,Minassian Angela M.,Lauffenburger Douglas A.,Seder Robert A.,Douek Daniel,Alter Galit,Draper Simon J.

Publisher

Elsevier BV

Subject

General Earth and Planetary Sciences,General Environmental Science

Reference89 articles.

1. Test samples were diluted in casein to a minimum dilution of 1:50 and 50�L was added in triplicate. Plates were incubated for 2 hours at 37�C and washed in washing buffer. An alkaline phosphatase-conjugated secondary antibody from Southern Biotech was diluted at the manufacturer's recommend minimum dilution for ELISA in casein. The antibody used was dependent on the isotype or subclass being assayed and were as follows: Mouse Anti-Human IgG1 Fc-AP (9054-04);Memory PfRH5-specific IgG+ B cells identified as live CD19+CD21+CD27+IgG+IgM-PfRH5/APC+PfRH5/PE+ lymphocytes unless otherwise specified (Supplemental Figure 1) and acquired on a Fortessa X20 flow cytometer with FACSDiva8.0 (both BD Biosciences,2020

2. Reduced blood-stage malaria growth and immune correlates in humans following RH5 vaccination;A M Minassian;Med (N Y),2021

3. Malaria Vaccines: Recent Advances and New Horizons;S J Draper;Cell Host Microbe,2018

4. Immunogenicity of standard and extended dosing intervals of BNT162b2 mRNA vaccine;R P Payne;Cell,2021

5. Reactogenicity and immunogenicity after a late second dose or a third dose of ChAdOx1 nCoV-19 in the UK: a substudy of two randomised controlled trials (COV001 and COV002);A Flaxman;Lancet,2021

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