Discovery and Functional Interrogation of the Virus and Host RNA Interactome of SARS-Cov-2 Proteins

Author:

Xiang Joy S.,Mueller Jasmine R.,Luo En-Ching,Yee Brian,Schafer Danielle,Schmok Jonathan C.,Tan Frederick E.,Her Hsuan-Lin,Chen Chun-Yuan,Brannan Kristopher W.,Jones Krysten L.,Park Samuel S.,Jin Wenhao,McVicar Rachael,Kwong Elizabeth M.,Le Phuong,Kofman Eric,Vu Anthony Q.,Li Yanhua,Tankka Alexandra T.,Dong Kevin D.,Song Yan,Carlin Aaron F.,Van Nostrand Eric L.,Leibel Sandra L.,Yeo Gene W.

Publisher

Elsevier BV

Reference197 articles.

1. Ratios are 611 normalized to the negative control (mean � s.e.m., n = 3 biologically independent replicate 612 transfections; * p<0.05, ** p<0.005, *** p<0.0005, **** p<0.0001, two-tailed multiple t-test; ns, 613 not significant). f) Bar plot showing the fold change of luciferase activity ratio and RT-qPCR 614 ratio (mean � s.e.m, n = 3; * p<0.05, *** p<0.001, two-tailed Welch's t-test). g) and h) Line 615 plots show the fold enrichment of eCLIP read coverage at each position on rRNAs for NSP1 (g, 616 blue) and ORF9c (h, blue), and the mean of 446 other RBPs deposited in the ENCODE 617 consortium;reporter RT-qPCR ratios (e) for the indicated coexpressed SARS-CoV-2 protein, known human 610 regulators of RNA stability (CNOT7, BOLL) and negative control

2. Electronic copy available at: https://ssrn.com/abstract=3867726 NSP12 eCLIP enriched RNA mapped to UGGT1, NDUFA4 and RPN1. g) Western blots 642 showing expression levels of UGGT1, NDUFA4 and RPN1, with ? actin as loading control, 643 from GFP or NSP12;Gene Ontology terms of NSP12 target genes. c)

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