Development of Robust and Reproducible Murine Brain Organoids Endowed With Networks of Functional Neurons and Specific Brain-Region Signature-Reference-Cited by-同舟云学术

Development of Robust and Reproducible Murine Brain Organoids Endowed With Networks of Functional Neurons and Specific Brain-Region Signature

Published:2021 Issue: Volume: Page:
ISSN:1556-5068
Container-title:SSRN Electronic Journal
language:en
Short-container-title:SSRN Journal

Author:

Ciarpella Francesca,Zamfir Raluca Georgiana,Campanelli Alessandra,Ren Elisa,Pedrotti Giulia,Bottani Emanuela,Caron Davide,Di Chio Marzia,Dolci Sissi,Ahtiainen Annika,Malpeli Giorgio,Malerba Giovanni,Bardoni Rita,Fumagalli Guido,Hyttinen Jari,Bifari Francesco,Palazzolo Gemma,Panuccio Gabriella,Curia Giulia,Decimo Ilaria

Publisher

Elsevier BV

Reference76 articles.

1. anti-ZBTB20 (rabbit, 1:200, Genetex, Cat#GTX121616), anti-KA1 (rabbit, 1:200, Abcam, Cat#ab67404), anti-NeuN (rabbit, 1:200, Genetex, Cat#GTX121616), anti-GAD65/67 (mouse, 1:400, Santa Cruz Biotechnology, Cat#sc-365180), anti-NMDA (mouse, 1:400, Santa Cruz Biotechnology, Cat#sc-365597), anti-GLSYN (mouse, 1:400, Santa Cruz Biotechnology, Cat#sc-74430), anti-GFAP (goat, 1:200, Abcam, Cat#ab53554), anti-synaptophysin (guinea pig, 1:200, Synaptic System, Cat#101004), anti-PSD95 (mouse, 1:200, Millipore, Cat#MAB1596), antigephyrin (mouse, 1:200, Synaptic Systems, Cat#147011), anti-VGAT (guinea pig, 1:400, Synaptic Systems, Cat#131004), anti-VGLUT (rabbit, 1:400, Synaptic Systems, Cat#135303), anti-Total OXPHOS Rodent WB Antibody Cocktail (mouse, 1:200, Abcam, ab110413 ), anti-VDAC1 (rabbit, 1:200, Cat# ab15895). Appropriate secondary antibodies were used: donkey anti-rabbit Alexa Fluor 488 (donkey, 1:1000, Thermo Fisher Scientific, Cat#A21206), donkey anti-mouse Alexa Fluor 488 (donkey, 1:1000;Antibodies The following primary antibodies were used: anti-SOX2 (goat, 1:200, R&D System, Cat#AF2018), anti-Vimentin (chicken 1:400, Millipore, Cat#AB5733), anti-Ki67 (rabbit, 1:200, Abcam, Cat#ab16667), anti-DCX (rabbit, 1:400, Cell Signaling Technology, Cat#4604), anti?3Tubulin (mouse, 1:400, Promega, Cat#G7121), anti-MAP2 (mouse, 1:200, Sigma-Aldrich, Cat#M1406),2120

2. Cat#A-11056), goat anti-rabbit CY3 (goat, 1:1000, Amersham, Cat#PA43004), donkey anti-rabbit Alexa Fluor 546 (donkey, 1:1000, Thermo Fisher Scientific, Cat#A10040), goat anti-chicken Alexa Fluor 546 (goat, 1:1000, Thermo Fisher Scientific, Cat#A11040);Amersham;Cat#PA43002), donkey anti-goat Alexa Fluor 546 (donkey, 1:1000, Invitrogen by Thermo Fisher Scientific

3. Cell Biology of Astrocyte-Synapse Interactions

4. Role of Mitochondrial Metabolism in the Control of Early Lineage Progression and Aging Phenotypes in Adult Hippocampal Neurogenesis

5. Innovative Human Three-Dimensional Tissue-Engineered Models As An Alternative To Animal Testing;P Bedard;Bioengineering,2020