1. Dorsal Forebrain regions where images in B were taken. (B) Representative images of sections with immunolabeling for Claudin11 (magenta) and E-cadherin (cyan) from various locations indicated in A, showing progressing appearance of arachnoid barrier cells from E12-E15;Quantification of biocytin-TMR assay in brain across ages when N=4 animals were injected with
2. Quantification of tracer fluorescence within the leptomeninges of E15 vs. E17 embryos from Claudin11+ arachnoid barrier cells 8 microns above the brain surface and down through the lower arachnoid and pia. Two-way Students T-Tests for 3C and 3D, with average of all arachnoid barrier or arachnoid and pia intensity values collapsed into one value for 3D, * = p < 0.05, ** = p < 0.01, *** = p < 0.001. Scale Bars are;B Alves De Lima;E15 and E17 biocytin-TMR injected embryos immunolabeled with Claudin 11 to show location of arachnoid barrier (AB) layer. (E),2020
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