1. Conclusion Based on the DNA-templated AgNCs and FRET-assisted signal amplification, a novel and ultrasensitive 3D DNM was developed for the quantitative determination of multiple miRNAs. AgNCs with DNA as a template endowed the 3D DNM with stable three branched 3-D stereo-structure and robust fluorescence luminescence signal intensity, especially in the presence of the fluorescence luminescence enhancer composed of FRET. Importantly, the 3D DNM provided high accuracy, high sensitivity and excellent storability. The proposed 3D DNM also achieved high selectivity for measuring miRNAs with LODs down to pmol L -1 level. On top of that, no 3D DNM was previously used in multiplexed miRNAs detection. Credit authorship contribution statement Wenxin Wang: Conceptualization, Methodology, Investigation, Data analysis and curation, Visualization, Writing-original draft;Methodology, Writing -Review and Editing. Li-Ping Jiang: Supervision, Resources, Validation, Funding acquisition, Writing-Review and Editing
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