1. mg/kg xylazine) and administered sustained release buprenorphine (0.5mg/kg, subcutaneous injection) prior to surgery. Information routing through the BLA was manipulated using chemogenetics to mimic or prevent the effects of CUS or EE: DREADD CUS: To mimic CUS, mice were stereotaxically injected with AAVrg-EF1a-Cre (Addgene #55636) in the NAc;AAVrg-hSyn-fDIO-hM3D(Gq)-mCherry-WPREpA (Addgene #154868) in the BnST
2. (Gi)-IRES-mCitrine (Addgene #50455) in the BLA (AP: -1.50; ML: +/-3.0; DV: -4.5) using a 33-gauge Hamilton syringe at an infusion rate of 100nL/minute. To prevent the effects of CUS, mice were injected with AAVrg-hSyn-fDIO-hM3D(Gq)-mCherry-WPREpA in the NAcc, AAVrg-EF1a-Cre in the BnST, and AAV1-EF1a-Flpo and AAV8-hSyn-DIO-HA;and AAV1-EF1a-Flpo
3. AAVrg-EF1a-Cre in the BnST, and AAV1-EF1a-Flpo and AAV8-hSyn-DIO-HA-hM4D(Gi)-IRES-mCitrine in the BLA. To prevent the effects of EE, mice were stereotaxically injected with AAVrg-EF1a-Cre in the NAcc, AAVrg-hSyn-fDIO-hM3D(Gq)-mCherry-WPREpA in the BnST, and AAV1-EF1a-Flpo and AAV8-hSyn-DIO-HA-hM4D(Gi)-IRES-mCitrine in the BLA and then subjected to the 3-week EE protocol. Following 3 weeks to allow for optimal DREADD expression and the conclusion of either the CUS or EE protocol, avoidance behaviors and stress-induced helplessness was assessed in these animals 30-min following an;Dreadd Ee;To mimic the effects of EE, mice were stereotaxically injected with AAVrg-hSyn-fDIO-hM3D(Gq)-mCherry-WPREpA in the NAcc
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5. Basolateral amygdala neurons facilitate reward-seeking behavior by exciting nucleus accumbens neurons;F Ambroggi;Neuron,2008