1. W115122006402 (24y/o M)] using the RosetteSep Human T cell enrichmnent cocktail kit [Stemcell]. Total T cells were washed twice with PBS and subsequently stained with 1�M Carboxyfluorescein succinimidyl ester (CFSE) in a waterbath at 37�C for 15 minutes. After staining, cells were washed twice with PBS, counted, and 0.1e6 cells resuspended in 0.1mL Aim V medium [ThermoFisher] supplemented with 10% human serum [R&D Systems] were either plated with moDC at ratios of 4:1 to 2048:1 (T cells to iDC);Hiasa;monocyte-derived macrophages (MDM) were gently dissociated from the plate using Cell Dissociation Buffer, enzyme-free, PBS (ThermoFisher), pelleted at 300xg for 5 minutes at RT, suspended in fresh MDM medium, and plated for experiments,2009
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