Associated of Variable Number Tandem Repeat (VNTR) Polymorphism in IL 4 with Susceptibility to Breast Cancer in Iraqi Women

Abstract

Background: Breast cancer (BC) is common disease in the worldwide. Furthermore genetic mutations are known to enhance BC risk. The aim of this research is to examine correlation between the frequency of BC among Iraqi women and a variable number tandem repeat (VNTR) polymorphism in the IL-4 gene.  Methods: The study included 74 patients and 54 healthy women. To determine the genotypes of the variable number of tandem repeats (VNTR) in the IL-4 gene, a Polymerase Chain Reaction (PCR) technique was employed. Additionally, a complete blood count (CBC) test was conducted. Logistic regression analyses were performed to evaluate the association between genotypes and the occurrence of BC. Measures of association were computed in the form of odds ratios (OR) and 95% confidence intervals (95% CI). The threshold for statistical significance in both CBC analysis and genotyping analysis was established as a p-value below 0.05. Results: Among the CBC analysis, only the results of RBC (red blood cell count) and WBC (white blood cell count) showed statistical significance at the p = 0.05 level when comparing the study population. However, our investigation identified no association between IL-4 gene variant genotypes and breast cancer in Iraqi women. Other than tumor differentiation and body mass index(p < 0.05), no clinical or pathological features of BC patients were linked to variant genotypes. Conclusion: The study concluded a substantial RBC and WBC relationship between patients and control. However, the IL-4 genetic variation does not appear to affect breast cancer development or progression. However, the IL-4 genetic variation may affect disease prognosis. Thus, more research is needed to determine how IL-4 genetic variation affects breast cancer prognosis.