A Comprehensive Review on Analytical Method Development using RP-HPLC and Recent Advances in Pharmaceutical Applications-Reference-Cited by-同舟云学术

A Comprehensive Review on Analytical Method Development using RP-HPLC and Recent Advances in Pharmaceutical Applications

Published:2023-04-11 Issue:2 Volume:2 Page:53-60
ISSN:2583-4053
Container-title:Journal for Research in Applied Sciences and Biotechnology
language:
Short-container-title:J. Res. Appl. Sci. Biotechnol.

Author:

Kumar Deepak,Kumar Amrendra,Kumar Vinay,Raj Arjesh,Rai Raja Ram Mohan,Baliyan Vishal,Kumar Nitish

Abstract

The analytical technique of choice for separating, identifying, and quantifying complex mixtures is high-performance liquid chromatography (HPLC). Reverse-phase liquid chromatography (RP-HPLC) is the preferred separation mode for high-performance liquid chromatography (HPLC) due to its adaptability and higher selectivity for hydrophobic compounds. This review article discusses the fundamentals of reversed-phase high-performance liquid chromatography (RP-HPLC). This covers the separation principle, various stationary and mobile phase types, and separation-affecting variables. This article highlights the need of developing and testing such methods in addition to outlining the advantages of using RP-HPLC in industries like pharmaceutical, food, and environmental analysis. As examples of more recent advancements in RP-HPLC, new stationary and mobile phases, RP-HPLC downsizing, and hyphenated methods are also discussed. This review article provides a comprehensive tool for designing, refining, and validating RP-HPLC processes.

Publisher

Stallion Publication

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