Impact of Immunohistochemical Expression of Collagen III and MMP-14 in Odontogenic Keratocyst

Abstract

Objective: Immunohistochemical expression of collagen III and MMP14 was evaluated in odontogenic keratocyst, and correlated tothe clinicopathological parameters to remark on the role of these markers in the biological behavior of the cyst.Methods: Twenty-three odontogenic keratocysts samples were collected from three pathological laboratories in Sulaimani.Demographic information and the available radiographic investigation were recorded. The immune expression of collagen III andMMP14 was evaluated and related to the clinicopathological variables. Chi-square test was used for analysis and a p-value of 0.05 wascut off point for significance.Results: Males were predominantly affected by odontogenic keratocysts lesions (69.6%), which were mainly detected in the >35 yearsage groups. Keratocyst lesions were seen more frequently in the mandible (82.6%), with well-defined borders, and unilocularappearance. Collagen III was oriented more commonly in a parallel direction (70.8%), with moderate intensity (45.8%). MMP14revealed cytoplasmic expression in the epithelial lining of the keratocyst (100%), score 3 was the most prevalent expression (54.2%)in the cystic wall, and prominent inflammation and epithelial separation were detected with high scoring of MMP14, which were(57.1%) and (56.3%) respectively.Conclusions: Both markers had independent combined roles in the cyst’s biological behavior, including the prevalent parallel direction of the collagen III, while overexpression of the MMP14 might have an impact role in the aggressive behavior of the lesion.