Stability of Emulsions and Nanoemulsions Stabilized with Biosurfactants, and their Antimicrobial Performance against Escherichia coli O157:H7 and Listeria monocytogenes

Abstract

Abstract Two novel biosurfactants – surfactin and its variant fatty acyl glutamic acid (FA-glu) – were compared with two commercial emulsifiers – lecithin, and a mixture of Tween 80 and lauric „arginate (TLA) – for formation and stability of emulsions and nano„emulsions containing cinnamaldehyde (CM). The nano„emulsions’/emulsions’ antimicrobial performance against two common foodborne pathogens Escherichia coli O157:H7 and Listeria monocytogenes was also compared. Two emulsifier concentrations (0.5% w/w and 1% w/w) and two homogenizing pressures (62.05 MPa and 124.10 MPa) were compared for emulsions droplet stability during storage for 46 days at 4°C, 25°C, and 37°C. Surfactin, FA-glu, and TLA resulted in formation of nanoemulsions at both concentrations, but lecithin did not. Droplet sizes did not change significantly during 38 days at stored temperatures for surfactin- and TLA- stabilized nano„emulsions. However, FA-glu and lecithin stabilized emulsions coalesced after Day 13 at 37°C; also, FA-glu stabilized emulsion thickened on the 38th day at 4°C. The incorporation of CM in nanoemulsions or emulsions did not lower the minimum inhibitory concentration (MIC) for two bacteria tested in broths. However, the CM nanoemulsions and emulsions showed enhanced effects in inhibiting bacterial growths at concentrations lower than MICs compared to non-emulfied CM, with more inhibition from nanoemulsions.