Determination of Melphalan by Micelle Enhanced Spectrofluorimetric Method: Application to Content Uniformity Testing and Human Plasma

Abstract

Abstract This manuscript describes a responsive, rapid, validated and economical spectrofluorimetric method for determination of Melphalan in its pharmaceutical dosage forms and spiked human plasma. The proposed method is relied on the investigation of fluorescence action of Melphalan in Tween-80 micellar system. The Melphalan shows strong native fluorescence at λem 365 nm upon excitation at λex 265 nm in Tween-80 system. There was good enhancement (1.68 fold) in fluorescence intensity in the presence of Tween-80. The proposed approach has a good linearity in the concentration range of 0.2 μg/mL–2 μg/mL. The lower limit of detection and that of quantitation was 0.03 μg/mL and 0.08 μg/mL respectively. The developed method has been validated and applied for content uniformity testing of Melphalan medicines available on the market and Melphalan spiked human plasma samples. The results are statistically compared and were found to be in good agreement to those obtained by comparison methods.