Micelles Activated Planar Chromatographic Separation of Hydrophilic Vitamins

Abstract

Abstract The separation efficiency of micellar solutions in thin layer chromatography of hydrophilic vitamins (B1, B9, B12 and C) has been examined using silica gel as stationary phase. The best results were obtained with mixed micelles (aqueous solution of anionic SDS (0.5%) plus non-ionic Triton X-100 (0.5%) surfactants) and butanol (9:1 v/v) as mobile phase. The proposed method is applicable to the identification and separation of vitamins (B1, B9 and C) present in drug samples (Becosule and Celin). The separation 1 μg of vitamin C from milligram quantities (up to 0.50 mg) of vitamin B1 has been successfully realized. Effect of metal ions as impurities on the separation of B1, B9 and vitamin C and limit of detection of these vitamins have been examined.