Development and Validation of Reverse Phase High Performance Liquid Chromatographic Method for Quantitative Estimation of Rasagiline Tablet Dosage Form

Abstract

In the present study, a reverse phase high performance liquid chromatographic method was developed and validated for the determination of Rasagiline mesylate in tablet dosage form with the use of selegiline as an internal standard. Chromatographic separation was carried out on Inert Sustain C18 (4.6 mm × 100 mm i.e., 3 µm particle size) column using a mobile phase consisting of water: acetonitrile: H3PO4 (80:20:0.1 % v/v). The flow rate was maintained at 1 ml and UV detection was measured at 211 nm. The calibration curve was linear over the range 2-18 µgml % R.S.D. The results of accuracy and precision studies were within the limits. The method was simple, rapid, and easy to apply, making it suitable for routine analysis of Rasagiline mesylate in tablet dosage form.