Quantitative analysis of bioactive compounds by LC-MS/MS from Inula graveolens

Abstract

Medicinal plants have been employed commonly in many countries to remedy of various ailments. In this study, Inula graveolens leaves (5.0 g) were extracted with methanol (100 mL) for 24 hours at room temperature. After filtration with Whatman 1 filter pater, the solvent was removed under reduced pressure to yield the crude extract. Quantification of natural products in methanol extract of Inula graveolens was determined by LC-MS/MS. Chlorogenic acid was determined as a chief product (3.5 mg/g extract). Furthermore, salicylic acid (0.055 mg/g extract), caffeic acid (0.054 mg/g extract), protocatechuic acid (0.026 mg/g extract), hesperidin (0.016 mg/g extract), rutin (0.0160 mg/g extract) were found in the methanol extract. Since Inula graveolens contains important bioactive compounds, it has the possibility to be used in pharmaceuticals and food.