Effect of acidified milk feeding on the intake, average daily gain and fecal microbiological diversity of Holsten dairy calves

Abstract

Objective: To evaluate the effect of feeding acidified milk on the growth and fecal microbial diversity of dairy calves.Methods: Twenty healthy 3-day-old female Holstein calves with similar body weights were selected and randomly divided into two groups. One group was fed pasteurized milk (PM, Control), while the other was fed acidified milk (AM) <i>ad libitum</i> until weaned (day 60). The experiment lasted until day 180.Results: There was no difference in the nutritional components between PM and AM. The numbers of <i>Escherichia coli</i> and total bacteria in AM were lower than in PM. At 31 to 40 and 41 to 50 days of age, the milk intake of calves fed AM was higher than that of calves fed PM (p<0.05), and the solid feed intake of calves fed AM was higher than that of calves fed PM at 61 to 90 days (p<0.05). The average daily gain of calves fed AM was also higher than that of calves fed PM at 31 to 60, 61 to 180, and 7 to 180 days (p<0.05). The calves fed AM tended to have a lower diarrhea rate than those fed PM (p = 0.059). <i>Bacteroides</i> had the highest abundance in the feces of calves fed AM on day 50, while <i>Ruminococcaceae_UCG_005</i> had the highest abundance in the feces of calves fed AM on day 90 and calves fed PM on days 50 and 90. At the taxonomic level, the linear discriminant analysis scores of 27 microorganisms in the feces of calves fed AM and PM on days 50 and 90 were higher than 4.0.Conclusion: Feeding AM increased calf average daily gain and affected fecal bacterial diversity.