Affiliation:
1. Department of Clinical Biochemistry
2. Endocrinology
3. Oncology Cambridge University Hospitals NHS Foundation Trust, Addenbrooke's Hospital, Cambridge, UK
Abstract
Background Antithyroglobulin antibodies are a prevalent cause of interference in serum thyroglobulin immunoassays. Current guidelines recommend that antithyroglobulin antibodies should be measured concurrently with thyroglobulin when monitoring thyroid cancer patients post‐thyroidectomy. However, the concordance between different antithyroglobulin assays has been questioned despite the availability of an international thyroglobulin antibody Reference Preparation. Methods Four antithyroglobulin assays currently in use in UK laboratories (Siemens Immulite®, Brahms GmbH, PerkinElmer AutoDELFIA and Siemens ADVIA Centaur®) were compared in a cohort of 145 thyroid cancer patients. Results Using reference data provided by the kit manufacturer, concordance between the assays was 74%. Adjusting the cut-offs to maximize agreement increased concordance to 90%. Recovery of exogenous thyroglobulin using the Brahms Tg-plus immunoradiometric assay was neither a specific nor a sensitive test for the presence of a positive antibody result by any assay. Conclusions Despite the availability of an international reference preparation, current antithyroglobulin assays show unacceptable variance.
Subject
Clinical Biochemistry,General Medicine
Cited by
24 articles.
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