PCR-based microsatellite analysis for differentiation and genetic monitoring of nine inbred SENCAR mouse strains-Reference-Cited by-同舟云学术

PCR-based microsatellite analysis for differentiation and genetic monitoring of nine inbred SENCAR mouse strains

Published:2001-04-01 Issue:2 Volume:35 Page:157-162
ISSN:0023-6772
Container-title:Laboratory Animals
language:en
Short-container-title:Lab Anim

Author:

Benavides F.¹,Glasscock E.¹,Coghlan L. G.²,Stern M. C.¹,Weiss D. A.²,Conti C. J.¹

Affiliation:

1. The University of Texas, M.D. Anderson Cancer Center, Science Park, Research Division, Smithville, TX

2. Science Park, Department of Veterinary Sciences, Bastrop, TX, 78602 USA

Abstract

Sixteen DNA microsatellites or simple sequence length polymorphisms (SSLPs), generated by polymerase chain reaction (PCR) were selected for use in the genetic quality control of the nine inbred SENCAR strains currently available. The SENCAR strains constitute a powerful tool for mechanistic studies of multi-stage skin carcinogenesis, as well as for studies to understand the underlying genetic basis of resistance to tumour promotion and progression. SSLP analysis is a fast and economical way for detecting genetic contamination (unexpected outcrosses) among these closely-related albino strains, where standard immunological and biochemical markers have been shown to be insufficient.

Publisher

SAGE Publications

Subject

General Veterinary,Animal Science and Zoology

Link

http://journals.sagepub.com/doi/pdf/10.1258/0023677011911534

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