Affiliation:
1. Department of Plastic Surgery, Neuroscience Research Center, Chang Zheng Hospital, The Second Military Medical University, Shanghai 200433, People's Republic of China
Abstract
A reliable animal model of facial nerve (FN) injury forms the basis for effective scientific studies of injury and repair of the FN. Currently, rodents are the most widely used animal model for such studies, most of which adopt a postauricular incision approach. However, it is difficult to carry out the procedure on perinatal rodents owing to characteristics of anatomy and the direction of incision. Based on anatomical studies on the extracranial segment of the FN in mice, we established a FN axotomy model by using a subauricular incision (SI) approach. The effect and the outcome of the FN transection were evaluated by electrophysiology, behavioral assessment and histological observation in 20 healthy four-week-old BALB/c mice. Favorable results were obtained in all of the mice and none died during the operation or subsequent observation period. Compared with the classic postauricular incision approach, the SI approach possesses multiple advantages including easier access, more satisfactory exposure of the FN trunk, minimal invasion and providing a larger operation field, which would be critical for those studies on the rodent in early development or delicate surgical manipulations.
Subject
General Biochemistry, Genetics and Molecular Biology
Cited by
8 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献