The 'normalization' of germ-free guineapigs with host-specific caecal microflora-Reference-Cited by-同舟云学术

The 'normalization' of germ-free guineapigs with host-specific caecal microflora

Published:1989-01-01 Issue:1 Volume:23 Page:48-52
ISSN:0023-6772
Container-title:Laboratory Animals
language:en
Short-container-title:Lab Anim

Author:

Boot R.¹,Koopman J. P.²,Kruijt B. C.³,Lammers R. M.⁴,Kennis H. M.²,Lankhorst A.⁵,Welling G. W.⁶,Hectors M. P. C.⁷

Affiliation:

1. Department of Animal Supply, Laboratory for Parasitology and Mycology, National Institute of Public Health and Environmental Protection, PO Box 1, 3720 BA Bilthoven, The Netherlands; Department for Laboratory Animal Bacteriology, Laboratory for Parasitology and Mycology, National Institute of Public Health and Environmental Protection, PO Box 1, 3720 BA Bilthoven, The Netherlands

2. Central Animal Laboratory, Catholic University, Nijmegen, The Netherlands

3. Department of Animal Supply, Laboratory for Parasitology and Mycology, National Institute of Public Health and Environmental Protection, PO Box 1, 3720 BA Bilthoven, The Netherlands

4. Department for Laboratory Animal Bacteriology, Laboratory for Parasitology and Mycology, National Institute of Public Health and Environmental Protection, PO Box 1, 3720 BA Bilthoven, The Netherlands

5. Department for Laboratory Animal Science, Faculty of Veterinary Medicine, State University, Utrecht, The Netherlands

6. Department of Medical Microbiology, State University, Groningen, The Netherlands

7. Department of Gastroenterology, Catholic University, Nijmegen, The Netherlands

Abstract

Hysterectomy-derived germ-free guineapigs were given colonization-resistant caecal flora from mice (mCRF) or microflora obtained from the caecum of an antibiotic-decontaminated conventional guineapig (gpCRF) and compared with guinea pigs raised conventionally with the sow. Body weight and the following intestinal parameters were determined for the groups: colonization resistance (CR) to Escherichia coli, relative caecal weight (RCW), β-aspartylglycine (faeces), volatile fatty adds (caecum) and bile acids (faeces). mCRF guineapigs showed values quite different from control animals for CR and RCW, indicating the unsuitability of mouse CRF for normalizing guineapigs. In gpCRF guineapigs CR and RCW values were comparable with controls, indicating the suitability of the guineapig flora for normalizing guineapigs. mCRF guineapigs housed with gpCRF guinea pigs, showed an improvement in CR and RCW, yielding values found in control animals.

Publisher

SAGE Publications

Subject

General Veterinary,Animal Science and Zoology

Link

http://journals.sagepub.com/doi/pdf/10.1258/002367789780886867

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