Comparison of media for isolation of mouse anaerobic faecal bacteria-Reference-Cited by-同舟云学术

Comparison of media for isolation of mouse anaerobic faecal bacteria

Published:1985-10-01 Issue:4 Volume:19 Page:353-358
ISSN:0023-6772
Container-title:Laboratory Animals
language:en
Short-container-title:Lab Anim

Author:

Itoh K.¹,Mitsuoka T.²

Affiliation:

1. Animal Physiology Laboratory, The Institute of Physical and Chemical Research, Wako-shi, Saitama, 35101, Japan; Department of Microbiology and Immunology, Louisiana State University Medical Center in Shreveport, 1501 Kings Highway, PO Box 33932, Shreveport, LA 71130-3932, USA

2. Animal Physiology Laboratory, The Institute of Physical and Chemical Research, Wako-shi, Saitama, 35101, Japan; Department of Biomedical Science, Faculty of Agriculture, The University of Tokyo, Bunkyo-ku, Tokyo, 113, Japan

Abstract

A study was made to determine suitable plate-in-bottle media for enumeration and isolation of the anaerobic faecal bacteria of mice. Comparison of non-selective media indicated that colony counts were higher on M-SM10, M10 and M-M98-5 than on EG, W & E medium and BHI, and those on M-SM10 were always the highest. Bacteroidaceae counts on M-SM10, M10, M-M98-5 were particularly high. On the other hand, in chloroform-treated faeces which contain only Clostridial spores, colony counts were higher on EG and W & E medium than on M-SM10, M10 and M-M98-5. These results indicate that suitable non-selective media vary according to the bacterial species to be enumerated and isolated.

Publisher

SAGE Publications

Subject

General Veterinary,Animal Science and Zoology

Link

http://journals.sagepub.com/doi/pdf/10.1258/002367785780887464

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