A method for estimating the concentration of spermatozoa in the rat cauda epididymidis-Reference-Cited by-同舟云学术

A method for estimating the concentration of spermatozoa in the rat cauda epididymidis

Published:1988-04-01 Issue:2 Volume:22 Page:154-156
ISSN:0023-6772
Container-title:Laboratory Animals
language:en
Short-container-title:Lab Anim

Author:

Kempinas W. G.¹,Lamano-Carvalho T. L.

Affiliation:

1. Department of Stomatology, Faculty of Dentistry of Ribeirão Preto, SP, Brazil

Abstract

A method for estimating the concentration of spermatozoa in the rat cauda epididymidis is described. Treatment of a sperm suspension with 0·05% collagenase for 20-60 min or 0·025% trypsin for 1-2 min at 34-37°C was found to result in consistently homogeneous sperm. Sperm concentration ranged from 152·5 to 230·0 × 107 spermatozoa/ml, with a mean of 187·7 (±5·6 SEM)×107 spermatozoa/mI.

Publisher

SAGE Publications

Subject

General Veterinary,Animal Science and Zoology

Link

http://journals.sagepub.com/doi/pdf/10.1258/002367788780864547

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