An enzyme-linked immunosorbent assay (ELISA) for monitoring antibodies to SP group Pasteurellaceae in guineapigs

Author:

Boot R.1,Thuis H.1,Bakker R. H. G.1,Veenema J. L.1

Affiliation:

1. Section of Laboratory Animal Microbiology, Laboratory for Parasitology and Mycology, National Institute of Public Health and Environmental Protection, PO Box 1, 3720 BA Bilthoven, The Netherlands

Abstract

Cross-reactivity studies with Pasteurellaceae from guineapigs revealed 5 serologically distinct groups, comprising Pasteurella multocida, Sp group bacteria, SP-like bacteria, Pasteurella pneumotropica and an actinobacillus-like bacterium. Guineapig Pasteurellaceae differed serologically from mouse-derived P. pneumotropica NCTC 8284. An enzyme-linked immunosorbent assay (ELISA) using SP group antigen, developed to monitor 'natural' infections by SP group Pasteurellaceae in guineapigs, detected significantly more infection than did cultivation, and was found superior to an ELISA performed with P. pneumotropica NCTC 8284.

Publisher

SAGE Publications

Subject

General Veterinary,Animal Science and Zoology

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3. Infectious Diseases;The Laboratory Rabbit, Guinea Pig, Hamster, and Other Rodents;2012

4. Classification of organisms previously reported as the SP and Stewart–Letscher groups, with descriptions of Necropsobacter gen. nov. and of Necropsobacter rosorum sp. nov. for organisms of the SP group;International Journal of Systematic and Evolutionary Microbiology;2011-08-01

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