Freezing rabbit semen by the use of BF5 diluent-Reference-Cited by-同舟云学术

Freezing rabbit semen by the use of BF5 diluent

Published:1990-04-01 Issue:2 Volume:24 Page:172-175
ISSN:0023-6772
Container-title:Laboratory Animals
language:en
Short-container-title:Lab Anim

Author:

Bamba K.¹,Adams C. E.²

Affiliation:

1. AFRC, Institute of Animal Physiology, Animal Research Station, 307 Huntingdon Road, Cambridge CB3 0JQ, UK; Faculty of Agriculture, Shizuoka University, Shizuoka 422, Japan

2. AFRC, Institute of Animal Physiology, Animal Research Station, 307 Huntingdon Road, Cambridge CB3 0JQ, UK

Abstract

Three experiments were carried out to find the optimal concentration of DMSO and glycerol in BFS diluent for freezing rabbit spermatozoa. Semen was diluted 1:1 with diluent A (BF5 + DMSO) at 25°C and diluted further 1:1 with diluent B (diluent A + glycerol) after cooling down to 5°C. Diluted semen was frozen immediately and stored in liquid nitrogen. Maximum percentages for motility and normal acrosomes were obtained in the presence of 12% DMSO (as expressed in diluent A) and 3% glycerol (final concentration) after thawing.

Publisher

SAGE Publications

Subject

General Veterinary,Animal Science and Zoology

Link

http://journals.sagepub.com/doi/pdf/10.1258/002367790780890149

Cited by 14 articles. 订阅此论文施引文献订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献

1. Proteomic analysis of rabbit fresh and cryopreserved semen provides an important insight into molecular mechanisms of cryoinjuries to spermatozoa;Theriogenology;2022-10

2. Synergistic effect of royal jelly in combination with glycerol and dimethyl sulfoxide on cryoprotection of Romanov ram sperm;Cryobiology;2022-02

3. Cryoprotection synergism between glycerol and dimethyl sulfoxide improves the mitochondrial transmembrane potential, plasmalemma, acrosomal and DNA integrities, and in vivo fertility of water buffalo (Bubalus bubalis) spermatozoa;Cytotechnology;2016-10-19

4. The Combinatorial Effect of Different Equex STM Paste Concentrations, Cryoprotectants and the Straw-Freezing Methods on the Post-Thaw Boar Semen Quality;Reproduction in Domestic Animals;2012-05-21

5. Cryopreservation of Iberian pig spermatozoa. Comparison of different freezing extenders based on post-thaw sperm quality;Animal Reproduction Science;2010-03