Injection technique and scanning electron microscopic study of the arterial pattern of the 20 gestation days (G20) rat fetus

Author:

Navarro M.1,Carretero A.1,Canut L.2,Perez-Aparicio F. J.1,Cristofol C.3,Manesse M.4,Sautet J.4,Arboix M.3,Ruberte J.1

Affiliation:

1. Department of Anatomy and Embryology, Veterinary Faculty, Autonomous University of Barcelona, 08193-Bellaterra, Spain

2. Laboratory of Teratology, Centro de Investigación y Desarrollo Aplicado, SAL 08130-Santa Perpetua de la Mogoda, Barcelona, Spain

3. Department of Pharmacology and Toxicology, Veterinary Faculty, Autonomous University of Barcelona, 08193-Bellaterra, Spain

4. Laboratory of Anatomy and Teratology, Ecole Nationale Vétérinaire, 23 Chemin des Capelles, F-31076 Toulouse, Cedex, France

Abstract

A technique to obtain microvascular corrosion casts of the G20 rat fetus and the normal pattern of the main arteries of the G20 rat fetus are described. The casts were studied by means of scanning electron microscopy (SEM). The arterial pattern is similar to that described in the adult; however, several variations have been found. It is concluded that the use of vascular corrosion casts studied by SEM may be particularly helpful to observe the extremely small arteries of rat fetuses. Moreover, we suggest that this technique may be useful in practical teratological studies.

Publisher

SAGE Publications

Subject

General Veterinary,Animal Science and Zoology

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