Inflammatory Status of Monocytes in Type 2 Diabetes Mellitus-Reference-Cited by-同舟云学术

Inflammatory Status of Monocytes in Type 2 Diabetes Mellitus

Published:2023-12-05 Issue:4 Volume:19 Page:25-34
ISSN:2713-0428
Container-title:Journal Biomed
language:
Short-container-title:BIOMEDICINE

Author:

Kirichenko T. V.¹,Bochkareva L. A.²,Nedosugova L. V.²,Markina Yu. V.¹,Kuzina I. A.²,Petunina N. A.²,Tolstik T. V.¹,Bogatyreva A. I.¹,Antonov V. A.³,Markin A. M.¹

Affiliation:

1. A.P. Avtsyn Research Institute of Human Morphology of B.V. Petrovsky National Research Centre of Surgery

2. I.M. Sechenov Moscow State Medical University (Sechenov University)

3. Research Institute of Hygiene, Toxicology and Occupational Pathology of the Federal Medical and Biological Agency of Russia

Abstract

Сhronic inflammation is considered as a key factor in the development of type 2 diabetes mellitus. Impaired tolerance of the inflammatory response of monocytes is regarded as an important mechanism in the pathogenesis of chronic inflammation. In this work, we study the inflammatory activation and tolerance of the immune response of monocytes in diabetes. In total, 40 patients with newly diagnosed diabetes and 40 control group participants were included in the study. The level of basal, LPS-stimulated and re-stimulated secretion of the TNF-α, IL-1β, and MCP-1 cytokines was assessed in a monocyte culture isolated from the blood by immunomagnetic separation of CD14+ cells. The level of basal, LPS-stimulated and re-stimulated TNF-α secretion was significantly higher in patients with diabetes; the level of IL-1β secretion did not differ significantly between the groups; basal and re-stimulated MCP-1 secretion was also significantly higher in the diabetes group. Re-stimulated secretion of TNF-α and IL-1β was reduced compared to primary-stimulated secretion in both groups, demonstrating the tolerance of the macrophage immune response to these cytokines. Re-stimulated secretion of MCP-1 in 42% of diabetes patients was higher than primary stimulated secretion, thus revealing an impaired tolerance of the immune response of macrophages. A correlation was found between TNF-α secretion and body mass index, r=0.631, p<0.001, and with glycemic level, r=0.427, p=0.037. The results obtained demonstrate inflammatory activation of monocytes with hypersecretion of TNF-α and MCP-1, impaired tolerance of the immune response of monocytes in diabetes regarding the secretion of MCP-1, as well as a correlation of TNF-α secretion with body mass index and glycemic level. This indicates an important role of TNF-α and MCP-1 in the pathogenesis of chronic inflammation in type 2 diabetes, thus allowing these cytokines to be considered as potential therapeutic targets for pathogenetic therapy of type 2 diabetes.

Publisher

Scientific Center for Biomedical Technologies of the Federal Medical-Biological Agency

Subject

General Medicine

Reference21 articles.

1. Al B., Suen T.K., Placek K., Netea M.G. Innate (learned) memory. J. Allergy Clin. Immunol. 2023;152(3):551–566. DOI: 10.1016/j.jaci.2023.06.014.

2. Alvarado-Vázquez P.A., Grosick R.L., Moracho-Vilrriales C., Ward E., Threatt T., Romero-Sandoval E.A. Cytokine production capabilities of human primary monocyte-derived macrophages from patients with diabetes mellitus type 2 with and without diabetic peripheral neuropathy. J. Pain Res. 2018;12:69–81. DOI: 10.2147/JPR.S186372.

3. Chen X., Zhang D., Li Y., Wang W., Bei W., Guo J. NLRP3 inflammasome and IL-1β pathway in type 2 diabetes and atherosclerosis: Friend or foe? Pharmacol. Res. 2021;173:105885. DOI: 10.1016/j.phrs.2021.105885.

4. de Baat A., Trinh B., Ellingsgaard H., Donath M.Y. Physiological role of cytokines in the regulation of mammalian metabolism. Trends Immunol. 2023;44(8):613– 627. DOI: 10.1016/j.it.2023.06.002.

5. Fujisaka S. The role of adipose tissue M1/M2 macrophages in type 2 diabetes mellitus. Diabetol. Int. 2020;12(1):74–79. DOI: 10.1007/s13340-020-00482-2.