Embryological Aspects of Creating a New Humanized Transgenic Mouse Line with an Integrated Human Gene <i>HLA A*02:01:01:01</i>-Reference-Cited by-同舟云学术

Embryological Aspects of Creating a New Humanized Transgenic Mouse Line with an Integrated Human Gene <i>HLA A*02:01:01:01</i>

Published:2022-12-11 Issue:4 Volume:18 Page:10-23
ISSN:2713-0428
Container-title:Journal Biomed
language:
Short-container-title:BIOMEDICINE

Author:

Savchenko E. S.¹,Ogneva N. S.¹,Karkischenko N. N.¹

Affiliation:

1. Scientific Center of Biomedical Technologies of the Federal Medical and Biological Agency of Russia

Abstract

The accumulation of scientific data in the field of pharmacogenetics requires the creation of adequate biomodels to reflect the immunogenetic characteristics of different population groups. We have obtained the ancestors of a new humanized transgenic mouse line carrying the human HLA-A*02:01:01:01 gene, which is characteristic of the Russian population. The new biomodels was created using the pronuclei microinjection method of a linearized fragment of genetically engineered DNA construct into zygotes, followed by overnight cultivation in CO2 incubator and transfer of potentially modified embryos at the stage of two 4.0 blastomere to pseudopregnant foster females. A total of 91 living offspring were obtained and analyzed, with 18 pups carrying the target genome modification. The resulting transgenic animals were used to create a new line of mouse biomodels carrying the human HLA-A*02:01:01:01 gene.

Publisher

Scientific Center for Biomedical Technologies of the Federal Medical-Biological Agency

Subject

General Medicine

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