Genotyping of mice lines for the TPH2 gene in routine practice: selection of a DNA extraction technique for PCR

Abstract

The development of a fast and highly accurate genotyping protocol is crucial for the use of biomodels with TPH2 gene knockout in experiments. Genotyping of mutant mice is currently carried out using methods that include sequencing or multi-stage PCR. These methods are expensive and time-consuming. This paper presents a comparative analysis of two methods: detection of amplification products by gel electrophoregram and real-time PCR. The procedure for extracting DNA from fragments of mice tails is also considered.