Author:
H Wien Nie ,Vu Thi Hao ,Tran Thi Hoai ,Mai Gia Thao ,Bui Cong Gia Bao ,Nguyen Khac Manh ,Thai Tien Dung
Abstract
This study introduces a new method to determine both TP and TN in one process, reducing the analytical cost and time. During this procedure, soil samples were digested in alkaline potassium persulfate (K2S2O8) at 150 °C for two hours in a reactor to convert organic N (including NH3/NH4+) and P species to the highest oxidation states NO3-, PO43-. After that, H2O2 removes the color of the digest, which could influence the efficiency of the analytical process. Finally, ions NO3- and PO43- were separated on the anion-exchange column and quantified by a conductivity detector. According to the spiked samples, which were made by mixing Bovine serum albumin (BSA) with organic nitrogen and triphenyl phosphate containing organic phosphorus with real soil matrix, the recovery of TN and TP was 85% and 97%, respectively. Both TN and TP linear concentration ranges were between 0.50 and 50 µg.mL-1. The results of relative standard deviation and limit of detection for TN were from 4.2%, and 0.31 mg.kg-1; and for TP were from 3.5%, and 0.25 mg.kg-1.
Publisher
Academy of Military Science and Technology
Reference21 articles.
1. [1]. Charles J.Potton. and Jennifer R.Kryskalla, “Methods of Analysis by the U.S. Geological Survey National Water Quality Laboratory—Evaluation of Alkaline Persulfate Digestion as an Alternative to Kjeldahl Digestion for Determination of Total and Dissolved Nitrogen and Phosphorus in Water”, U.S. Geological Survey WaterResources Investigations Report 03–4174, Denver,CO, (2003).
2. [2]. Hagedorn, Frank & Schleppi, Patrick. “Determination of total dissolved nitrogen by persulfate oxidation”, Journal of Plant Nutrition and Soil Science - J PLANT NUTR SOIL SCI. 163. 81-82, (2000).
3. [3]. John D.Pfaff. “Method 300.0: Determination of inorganic anions by ion chromatography”, U.S. EPA, (1993).
4. [4]. https://www.sigmaaldrich.com/VN/en/technical-documents/technical-article/cell-culture-and-cell-culture-analysis/cell-culture-media-preparation/albumin-from-bovine-serum.
5. [5]. Lakesh K. Sharma et al. "Improving Nitrogen and Phosphorus Efficiency for Optimal Plant Growth and Yield" In New Visions in Plant Science, edited by Özge Çelik. London: IntechOpen, (2017).