Myosin Va facilitates the distribution of secretory granules in the F-actin rich cortex of PC12 cells
Author:
Rudolf Rüdiger1, Kögel Tanja1, Kuznetsov Sergei A.12, Salm Thorsten1, Schlicker Oliver1, Hellwig Andrea1, Hammer John A.3, Gerdes Hans-Hermann1
Affiliation:
1. Department of Neurobiology, Interdisciplinary Center of Neuroscience,University of Heidelberg, Im Neuenheimer Feld 364, D-69120 Heidelberg,Germany 2. Present address: Institute of Cell Biology and Biosystems Technology,University of Rostock, Albert-Einstein Str. 3, D-18051 Rostock, Germany 3. Laboratory of Cell Biology, National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, MD 20892, USA
Abstract
Neuroendocrine secretory granules, the storage organelles for neuropeptides and hormones, are formed at the trans-Golgi network, stored inside the cell and exocytosed upon stimulation. Previously, we have reported that newly formed secretory granules of PC12 cells are transported in a microtubule-dependent manner from the trans-Golgi network to the F-actin-rich cell cortex, where they undergo short directed movements and exhibit a homogeneous distribution. Here we provide morphological and biochemical evidence that myosin Va is associated with secretory granules. Expression of a dominant-negative tail domain of myosin Va in PC12 cells led to an extensive clustering of secretory granules close to the cell periphery, a loss of their cortical restriction and a strong reduction in their motility in the actin cortex. Based on this data we propose a model that implies a dual transport system for secretory granules: after microtubule-dependent delivery to the cell periphery, secretory granules exhibit a myosin Va-dependent transport leading to their restriction and even dispersal in the F-actin-rich cortex of PC12 cells.
Publisher
The Company of Biologists
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