The distribution of mitochondria and endoplasmic reticulum in relation with secretory sites in chromaffin cells

Abstract

The distribution of mitochondria and ER in relation to exocytotic sites is relevant to understand the influence of these organelles in tuning calcium signals and secretion. Confocal images of probes tagged to mitochondria and F-actin cytoskeleton revealed the existence of two populations of mitochondria, one cortical and the other perinuclear. This mitochondrial distribution was also confirmed by using electron microscopy. In contrast, ER was sparse in the cortex and more abundant in deep cytoplasmic regions. The mitochondrial distribution may be due to organellar transport, which experiences increasing restrictions in the cell cortex. Further study of organelle distribution in relation to SNARE microdomains or the granule fusion sites revealed that 1/3 of the cortical mitochondria co-localized with exocytotic sites whereas another 1/3 located at a distance smaller than 2 vesicle diameters. ER structures were also present in the vicinity of secretory sites but at a lower density. Therefore, mitochondria and ER have a spatial distribution that suggests a specialized role in modulation of exocytosis and fits with cytosolic Ca2+ microdomains described before.