Drosophila I-Rhybrid dysgenesis is associated with catastrophic meiosis and abnormal zygote formation

Author:

Orsi Guillermo A.1,Joyce Eric F.2,Couble Pierre1,McKim Kim S.2,Loppin Benjamin1

Affiliation:

1. Centre de Génétique Moléculaire et Cellulaire, CNRS UMR5534, University of Lyon, UCBL Lyon1, Villeurbanne, F-69100, France

2. Waksman Institute and Department of Genetics, Rutgers University, 190, Frelinghuysen Rd, Piscataway NJ 08854, USA

Abstract

The Drosophila I-R type of hybrid dysgenesis is a sterility syndrome (SF sterility) associated with the mobilization of the I retrotransposon in female germ cells. SF sterility results from a maternal-effect embryonic lethality whose origin has remained unclear since its discovery about 40 years ago. Here, we show that meiotic divisions in SF oocytes are catastrophic and systematically fail to produce a functional female pronucleus at fertilization. As a consequence, most embryos from SF females rapidly arrest their development with aneuploid or damaged nuclei, whereas others develop as non-viable, androgenetic haploid embryos. Finally, we show that, in contrast to mutants affecting the biogenesis of piRNAs, SF egg chambers do not accumulate persistent DNA double-strand breaks, suggesting that I-element activity might perturb the functional organization of meiotic chromosomes without triggering an early DNA damage response.

Publisher

The Company of Biologists

Subject

Cell Biology

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