Plasticity in gastrointestinal morphology and enzyme activity in lactating striped hamsters (Cricetulus barabensis)

Abstract

In small mammals marked phenotypic plasticity of digestive physiology has been shown to make it easier to cope with the energetically stressful periods, such as lactation. It has been proposed that the capacity of the gut to digest and absorb food is not the factor limiting to sustained energy intake (SusEI) during peak lactation. In this study, plasticity in energy intake and gastrointestinal morphology was examined in striped hamsters at different stages of reproduction and raising litters of different sizes. Mechanisms associated with digestive enzymes and neuroendocrine hormones underpinning the plasticity were also examined. The females significantly increased energy intake, digestibility, masses of digestive tracts and activity of stomach pepsin and maltase, sucrase and aminopeptidase of small intestine in peak lactation compared to the non-productive and post-lactating periods. Further, the females raising large litters significantly increased energy intake, digestibility, gastrointestinal mass and activity of digestive enzymes, and weaned heavier offspring compared with those nursing small and medium litters, indicating that the significant plasticity of digestive physiology increased reproductive performance. The agouti-related protein (AgRP) mRNA expression in the hypothalamus was up-regulated significantly in the females raising large litters relative to those raising small litters. Serum leptin levels, hypothalamus neuropeptide Y (NPY), or anorexigenic neuropeptides (pro-opiomelanocortin / cocaine- and amphetamine-regulated transcript, POMC / CART) mRNA expression did not differ among the females raising small, medium and large litters, indicating that leptin levels in lactation might only reflect a state of energy balance rather than being the prime driver of hyperphagia. Some hypothalamic neuropeptides, such as NPY, POMC and CART, would be involved in the limits to the SusEI during lactation.