Affiliation:
1. Zoology Department, Marischal College, University of Aberdeen, and Animal Breeding and Genetics Research Organization, Institute of Animal Genetics Edinburgh
Abstract
1. Methods are described for the aseptic culture of Drosophila.
2. All the factors necessary for normal development can be extracted from yeast water-soluble form.
3. A chemically defined medium sufficient for almost normal growth is described and includes: casein (and gelatin) as a source of amino-acids, dextrose, cholesterol, ergosterol, yeast nucleic acid, inositol, biotin, aneurin hydrochloride, riboflavine, nicotinic acid, pyridoxin hydrochloride, Ca-pantothenate, choline chloride, thymine and folic acid.
4. For completely normal development an alkali-soluble fraction must be added. This fraction shows nucleoprotein-like reaction, but it is neither arginine nor nucleic acid.
5. Folic acid is essential for pupation.
6. Attention is drawn to the need for caution in gene-action studies with synthetic media which do not give completely normal development even in the wild type.
Publisher
The Company of Biologists
Subject
Insect Science,Molecular Biology,Animal Science and Zoology,Aquatic Science,Physiology,Ecology, Evolution, Behavior and Systematics
Cited by
14 articles.
订阅此论文施引文献
订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献