Adenosine receptor blockade and hypoxia-tolerance in rainbow trout and Pacific hagfish. II. Effects on plasma catecholamines and erythrocytes

Abstract

The purpose of this study was to examine the role of adenosine receptors (ARs) in (1) the regulation of catecholamine secretion and (2) the modulation of blood oxygen capacitance by catecholamines. To this end, we assessed the response of rainbow trout and Pacific hagfish treated with either an AR blocker, theophylline, or saline under hypoxic and normoxic conditions. Compared with the control hypoxic rainbow trout, AR blockade resulted in a smaller increase in haematocrit and haemoglobin (Hb) concentration of the blood, smaller red blood cell transmembrane pH differences and mean cellular [Hb] (MCHC), as well as a 16-fold higher plasma adrenaline concentration after only 10 min of acute hypoxic exposure. In hypoxic hagfish, AR blockade had no effect on the [Hb] of the blood, and there was no regulation of red blood cell pH or changes in MCHC. However, whereas plasma [adrenaline] did not change following exposure to a PwO2 of 1.33 kPa in the hypoxic sham group, the concentration increased 3.8-fold within 10 min in the theophylline-injected group. These results suggest that adenosine modulates the circulating level of catecholamines in both hypoxic rainbow trout and hypoxic Pacific hagfish.