RBOH-mediated ROS production facilitates lateral root emergence in Arabidopsis

Author:

Orman-Ligeza Beata123ORCID,Parizot Boris23,de Rycke Riet23,Fernandez Ana23,Himschoot Ellie23,Van Breusegem Frank23ORCID,Bennett Malcolm J.4ORCID,Périlleux Claire5,Beeckman Tom23,Draye Xavier1ORCID

Affiliation:

1. Université catholique de Louvain, Earth and Life Institute, Louvain-la-Neuve, Belgium

2. Department of Plant Biotechnology and Bioinformatics, Ghent University, B-9052, Ghent, Belgium

3. Department of Plant Systems Biology, VIB, B-9052, Ghent, Belgium

4. Centre for Plant Integrative Biology, School of Biosciences, University of Nottingham, Sutton Bonington, LE12 5RD, United Kingdom

5. PhytoSYSTEMS, Laboratory of Plant Physiology, University of Liège, Sart Tilman Campus, 4 Chemin de la Vallée, B-4000 Liège, Belgium

Abstract

Lateral root (LR) emergence represents a highly coordinated process in which the plant hormone auxin plays a central role. Reactive oxygen species (ROS) have been proposed to function as important signals during auxin-regulated LR formation, however their mode of action is poorly understood. Here, we report that Arabidopsis roots exposed to ROS show increased LR numbers due to the activation of LR pre-branch sites and LR primordia (LRP). Strikingly, ROS treatment can also restore LR formation in pCASP1:shy2-2 and aux1 lax3 mutant lines in which auxin-mediated cell wall accommodation and remodeling in cells overlying the sites of LR formation is disrupted. Specifically, ROS are deposited in the apoplast of these cells during LR emergence, following a spatio-temporal pattern that overlaps the combined expression domains of extracellular ROS donors of the RESPIRATORY BURST OXIDASE HOMOLOGS (RBOH). We also show that disrupting (or enhancing) expression of RBOH in LRP and/or overlying root tissues decelerates (or accelerates) the development and emergence of LRs. We conclude that RBOH-mediated ROS production facilitates LR outgrowth by promoting cell wall remodeling of overlying parental tissues.

Funder

Fonds Wetenschappelijk Onderzoek Vlaanderen

EURoot: Enhancing resource Uptake from roots under Stress in Cereal Crops

Communauté française de Belgique

Interuniversity Attraction Poles Programme initiated by the Belgian Science Policy Office

Fonds pour la Formation à la Recherche dans lIndustrie et dans lAgriculture

Publisher

The Company of Biologists

Subject

Developmental Biology,Molecular Biology

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3