Characterising multi-level effects of an acute pressure exposure on a shallow-water invertebrate: insights into the kinetics and hierarchy of the stress response

Abstract

Hydrostatic pressure is an important, ubiquitous, environmental variable of particular relevance in the marine environment. However, it is widely overlooked despite recent evidence that some marine ectotherms may be demonstrating climate-driven bathymetric range shifts. Wide ranging effects of increased hydrostatic pressure have been observed from the molecular level through to the behavioural level. Still, no study has simultaneously examined these multiple levels of organisation in a single experiment in order to understand the kinetics, hierarchy, and interconnected nature of such responses during an acute exposure, and over a subsequent recovery period. Herein, we quantify the transcription of a set of previously characterised genes during, and following, an acute pressure exposure in adults of the shrimp Palaemonetes varians. Further, we perform respiratory rate and behavioural analysis over the same period. Increases in gene expression were observed during, and following, exposure in genes associated with stress and metabolism. Respiratory rate increased during exposure, and into the recovery period. Finally, differential behaviour was observed under elevated hydrostatic pressure in comparison to ambient pressure. Characterising generalised responses to acute elevated pressure is a vital pre-cursor to longer–term, acclimation-based pressure studies. Results provide a novel insight into what we term the overall stress response (OSR) to elevated pressure; a concept that we suggest to be applicable to other environmental stressors. We highlight the importance of considering more than a single component of the stress response in physiological studies, particularly in an era where environmental multi-stressor studies are proliferating.