Distinct actin cytoskeleton behaviour in primary and immortalised T-cells

Author:

Colin-York Huw1ORCID,Kumari Sudha2,Barbieri Liliana1ORCID,Cords Lena1,Fritzsche M.13

Affiliation:

1. MRC Human Immunology Unit, Weatherall Institute of Molecular Medicine, University of Oxford, Headley Way, OX3 9DS Oxford, UK

2. Wolfson Imaging Centre Oxford, Weatherall Institute of Molecular Medicine, University of Oxford, Headley Way, OX3 9DS Oxford, UK; Massachusetts Institute of Technology, Cambridge, 02139, USA

3. Kennedy Institute for Rheumatology, Roosevelt Drive, University of Oxford, Oxford OX3 7LF Oxford, UK

Abstract

Cytoskeletal actin dynamics are crucial for the activation of T-cells. Immortalised Jurkat T-cell lines have been the model system of choice to examine and correlate the dynamics of the actin cytoskeleton and the immunological synapse leading to T-cell activation. However, it has remained unclear whether the immortalised cellular systems such as Jurka T-cells can recapitulate the primary T-cells’ cytoskeletal behaviour. Studies delineating the cytoskeletal behaviour of Jurkat T-cells in comparison to primary T-cells are lacking. Here, we employ live-cell super-resolution microscopy to investigate the cytoskeletal actin organisation and dynamics of living primary and immortalised Jurkat T-cells at the right spatiotemporal resolution. At comparable activation conditions, we found differences in the architectural organisation and dynamics of Jurkat and primary Mouse and Human T-cells. While the three major actin network architectures in Jurkat T-cells were reminiscent of primary T-cells, there was a difference in the organisation as well as molecular mechanisms of these networks. Our results highlight mechanistic distinctions in the most-utilised T-cell model system to study cytoskeletal actin dynamics.

Funder

Wellcome Trust

Engineering and Physical Sciences Research Council

Publisher

The Company of Biologists

Subject

Cell Biology

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3