Affiliation:
1. Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720, USA
Abstract
During Xenopus gastrulation, Wnt and FGF signaling pathways cooperate to induce posterior structures. Wnt target expression around the blastopore falls into two main categories: a horseshoe shape with a dorsal gap, like Wnt8 expression, or a ring, like FGF8 expression. Using ChIPseq, we show, surprisingly, that FGF signaling mediator, Ets2, binds near all Wnt target genes. However, β-catenin preferentially binds at the promoters of genes with horseshoe patterns, but further from the promoter of genes with ring patterns. Manipulation of FGF or Wnt signaling demonstrated that “ring” genes are responsive to FGF signaling at the dorsal midline while “horseshoe” genes are predominantly regulated by Wnt signaling. We suggest that in the absence of active β-catenin at the dorsal midline the DNA binding protein TCF binds and actively represses gene activity only when close to the promoter. In contrast, genes without functional TCF sites at the promoter may be predominantly regulated by Ets at the dorsal midline and are expressed in a ring. These results suggest recruitment of only short-range repressors to potential Wnt targets in the Xenopus gastrula.
Funder
National Institutes of Health
Publisher
The Company of Biologists
Subject
Developmental Biology,Molecular Biology
Cited by
17 articles.
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